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Isolation of a branched-chain α-keto acid decarboxylase from rat liver

✍ Scribed by Eccleston A. Kean; Errol Y.St.A. Morrison


Book ID
115735136
Publisher
Elsevier Science
Year
1979
Weight
331 KB
Volume
567
Category
Article
ISSN
0005-2744

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Purification of branched chain α-ketoaci
✍ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s