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Purification and characterization of human liver branched-chain α-keto acid dehydrogenase complex

✍ Scribed by K. Ono; M. Hakozaki; H. Nishimaki; H. Kochi

Book ID
113382592
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
737 KB
Volume
37
Category
Article
ISSN
0885-4505

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Purification of branched chain α-ketoaci
Purification of branched chain α-ketoacid dehydrogenase complex from rat liver
✍ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s