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Isolation and sequence of theHOG1 homologue fromDebaryomyces hansenii by complementation of thehog1? strain ofSaccharomyces cerevisiae

✍ Scribed by Bansal, Parmil K.; Mondal, Alok K.

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 190 KB
Volume: 16
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(20000115)16:1<81::aid-yea510>3.0.co;2-i

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✦ Synopsis

The HOG1 gene encodes a MAP kinase that plays an essential role in maintaining water homeostasis in the yeast Saccharomyces cerevisiae. A gene homologous to S. cerevisiae HOG1 has been isolated from a highly salt-tolerant yeast, Debaryomyces hansenii, by phenotypic complementation. DNA sequencing of the clone revealed the presence of an open reading frame encoding a protein 387 amino acids long. The deduced amino acid sequence showed very high similarity with homologous genes identi®ed from S. cerevisiae, Candida albicans and Zygosaccharomyces rouxii. In addition, it has also TGY motif characteristics of hyperosmolarity-activated MAP kinases.

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