✦ LIBER ✦

Isolation and separation of cells from the digestive gland epithelium ofBusycon canaliculatum

✍ Scribed by Cornell-Bell, A. H. ;Munro, D. R.

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 991 KB
Volume: 219
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402190305

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A structural analysis of the intact digestive gland epithelium of Busycon canaliculatum was undertaken at the light and ultrastructual levels. Procedures were developed for the isolation and separation of component epithelial cells by modifying existing pronase digestion techniques. Three distinct cell types were identified following separation by velocity sedimentation. Cells identified after isolation, and separation can be related to the intact digestive gland epithelium.

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