Separation and identification of the carotenoid pigments of stigmata isolated from light-grown cells ofEuglena gracilisstrain Z
✍ Scribed by Diana V. Heelis; William Kernick; Glyn O. Phillips; Keith Davies
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 444 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Thin layer chromatography of the carotenoid pigments of stigmata isolated from light grown cells of Euglena gracilis strain Z resolved 29 compounds, of which 16 could be eluted and their absorption spectra recorded. Seven of these compounds were identified by a combination of co-chromatography with authentic compounds and by chemical tests, one of these compounds (fl-carotene) was further identified by mass spectrometry. The major carotenoids were found to be fi-carotene, diatoxanthin and diadinoxanthin which together comprised approximately 60 ~o of the stigma pigments. In addition significant quantities of canthaxanthin, echinenone and cryptoxanthin were isolated and a possible carotenoid ester was detected. The results of this analysis are compared with those of previous workers and the significance of the findings is discussed.