Isolation of rat Kupffer cells: a combin
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V. Valatas; C. Xidakis; H. Roumpaki; G. Kolios; E.A. Kouroumalis
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Article
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2003
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Elsevier Science
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English
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## Abstract We report a fourβstep procedure that optimizes the methodology for isolation of highly purified rat Kupffer cells (KC). We combined the previously reported techniques of enzymatic tissue treatment, density gradient centrifugation, centrifugal elutriation and selective adherence. EDβ2 im