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Isolation and primary culture of rat Kupffer cells

✍ Scribed by JOHN K OLYNYK; SHARON L CLARKE


Book ID
108949685
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
401 KB
Volume
13
Category
Article
ISSN
0815-9319

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## Abstract We report a four‐step procedure that optimizes the methodology for isolation of highly purified rat Kupffer cells (KC). We combined the previously reported techniques of enzymatic tissue treatment, density gradient centrifugation, centrifugal elutriation and selective adherence. ED‐2 im