Isolation and kinetic properties of the larval brain phosphofructokinase from the gypsy moth, Lymantria dispar
β Scribed by Algimantas P. Valaitis
- Book ID
- 113324371
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 835 KB
- Volume
- 106
- Category
- Article
- ISSN
- 1096-4959
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A peptide (Lymantria TE) was isolated from brains of the gypsy moth, Lymantria dispar, which stimulates synthesis of ecdysteroid in the testes of larval and pupal insects. This ecdysiotropic peptide was purified and its structure determined to be NH 2 -Ile-Ser-Asp-Phe-Asp-Glu-Tyr-Glu-Pro-Leu-Asn-Asp
Normal and reverse-phase high-performance liquid chromatography in conjunction with radioimmunoassay and mass spectrometry were used to identify the free and conjugated ecdysteroids (after enzymatic hydrolysis) from day-4 pupae of the gypsy moth, Lymantria dispar L. Seven ecdysteroids were searched