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Identification of 20-hydroxyecdysone and ecdysone from the pupa of the gypsy moth, Lymantria dispar

โœ Scribed by M. F. Feldlaufer; W. R. Lusby; J. A. Svoboda; M. J. Thompson

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
482 KB
Volume
1
Category
Article
ISSN
0739-4462

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โœฆ Synopsis

Normal and reverse-phase high-performance liquid chromatography in conjunction with radioimmunoassay and mass spectrometry were used to identify the free and conjugated ecdysteroids (after enzymatic hydrolysis) from day-4 pupae of the gypsy moth, Lymantria dispar L. Seven ecdysteroids were searched for, but only 20-hydroxyecdysone (964 nglg fresh weight) and ecdysone (367 nglg fresh weight) were detected. Analysis of conjugated ecdysteriods after liberation by hydrolysis also indicated the presence of 20hydroxyecdysone (21.6 nglg fresh weight) and ecdysone (2.4 nglg fresh weight). Neither 26-hydroxyecdysone nor the 3a-epimers of 20-hydroxyecdysone or ecdysone were detected.

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