Methyl 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-hepto+ ++ pyranoside (1) was released from the lipopolysaccharide of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli by methanolysis and isolated by high-voltage paper electrophoresis. Its chemical structure was de
Isolation and identification of 3-deoxy-d-threo-hexulosonic acid as a constituent of the lipopolysaccharide of Vibrio parahaemolyticus serotypes O7 and O12
✍ Scribed by Seiichi Kondo; Ulrich Zähringer; Ernst Th. Rietschel; Kazuhito Hisatsune
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 549 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Treatment of the lipapolysaccharide of Vibrioparahaemolyticus serotypes 07 arid 012 with mild acid gave a compound which, on the basis of chemical analysis, g.l.c.-m.s., and 'H and 13C-n.m.r. spectroscopy, was characterized as 3-deoxy-Dthree-hexulosonic acid and shown to be present as a terminal non-substituted pyranose unit.
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Recently, we reported' on the occurrence of a normal mouse serum protein which binds specifically to the heptose/3-deoxy-o-manno-octulosonate (KDO) region of all lipopolysaccharides (LPS) studied. Immunochemical investigations indicated that the minimal carbohydrate structure required for binding in