Isolation and identification of a rhamnosyl-rhamnosyl-3-deoxy-d-manno-octulosonic acid trisaccharide from the lipopolysaccharide of Acinetobacter calcoaceticus (10303 NTCC London)

✍ Scribed by Helmut Brade; Angelika Tacken; Rudolf Christian

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 403 KB
• Volume: 167
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(87)80287-2

✦ Synopsis

Recently, we reported' on the occurrence of a normal mouse serum protein which binds specifically to the heptose/3-deoxy-o-manno-octulosonate (KDO) region of all lipopolysaccharides (LPS) studied. Immunochemical investigations indicated that the minimal carbohydrate structure required for binding included KDO and a neutral sugar attached thereto. The stereochemistry of the neutral sugar did not seem to contribute to the specificity of binding, since LPS containing L-glycero-D-manno-heptopyranose1~2, o-mannopyranose2, and D-glucopyranose3v4 were equally active in the interaction with the above-mentioned protein. However, the neutral sugar was always linked5 to position 5 of KDO.

During these studies, we found an LPS in a strain of Acinefobacter calco-