Isolation and chemical analysis of 7-O-(2-amino-2-deoxy-α-d-glucopyranosyl)-l-glycero-d-manno-heptose as a constituent of the lipopolysaccharides of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli and Vibrio cholerae
✍ Scribed by Wieslav Kaca; Jacqueline de Jongh-Leuvenink; Ulrich Zähringer; Ernst Th. Rietschel; Helmut Brade; Jan Verhoef; Volker Sinnwell
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 649 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
Methyl 7-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-L-glycero-D-manno-hepto+ ++ pyranoside (1) was released from the lipopolysaccharide of the UDP-galactose epimerase-less mutant J-5 of Escherichia coli by methanolysis and isolated by high-voltage paper electrophoresis. Its chemical structure was determined by chemical analysis, deamination with nitrous acid, g.1.c.-m.s., and 1H- and 13C-n.m.r. spectroscopy performed on its acetylated derivative. The disaccharide moiety of 1 was also detected in lipopolysaccharides of Vibrio cholerae.