𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and determination of porphyrins and their precursors in tissue

✍ Scribed by Olga Gutniak; Edward Kowalski

Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
406 KB
Volume
16
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Separation of porphyrins present in the autopsy organs of humans and animals with porphyria or in in vitro systems for studies of porphyrin metabolism is hampered by difficulties due to the physicochemical similarities between the individual components. The conventional techniques employed for the analysis and isolation of porphyrins (1, 2) are timeconsuming and cause serious losse s of material. In this paper a method is described which permits separation and determination of porphyrins and their precursors in a single sample of tissue. Of further interest is the separation of PBG' from Cro and the isolation of both of them from the tissue in the early course of the procedure.

MATERIALS

Water: twice distilled. Purification of solvents: chloroform, methanol, dioxane, and glacial acetic acid, according to Falk ( 1). Other solvents used were of reagent grade except for ethyl acetate after distillation and sodium acetate after recrystallization.

Spectrophotometer: Unicam SP-500 (1 cm cuvct). Chromatography paper: Whatman Xo. 1. Standard reference substances: (a) Copro III-tetramethyl ester obtained from Corynebacterium diphtheriae according to Gray and Holt (5). (b) Copro I, Copro III, Cro I, Vro III-methyl esters isolated from specimens of PCT urines according to Gutniak (6). (c) Mixture of Uro I + III-octamethyl esters prepared from PBG by heating human PA1 urines according to Gutniak (7).

📜 SIMILAR VOLUMES

ChemInform Abstract: Spectral Properties
ChemInform Abstract: Spectral Properties of Porphyrins and Their Precursors and Derivatives.
✍ N. Zh. Mamardashvili; O. A. Golubchikov 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 24 KB 👁 2 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

Polymer-bound porphyrins and their precu
Polymer-bound porphyrins and their precursors, 11. Syntheses and polymerization of methacryloyloxy and 2,4-hexadienoyloxy derivatives of porphyrins and phthalocyanines
✍ Holger Eichhorn; Michael Sturm; Dieter Wöhrle 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 827 KB

## Abstract The syntheses of tetraphenylporphyrins 1b, d, e containing four methacryloyloxy or 2,4‐hexadienoyloxy substituents and phthalocyanines 2b, d containing four methacryloyloxy substituents are described. Copolymerizations with styrene in solution were investigated in detail, and the compos

Identification and characterization of n
Identification and characterization of neuronal precursors and their progeny from human fetal tissue
✍ David R. Piper; Tahmina Mujtaba; Hansoo Keyoung; Neeta S. Roy; Steven A. Goldman 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 923 KB

## Abstract We have examined primary human neuronal precursors (HNPs) from 18–22‐week‐old fetuses. We showed that E‐NCAM/MAP2/β‐III tubulin‐immunoreactive neuronal precursors divide in vitro and could be induced to differentiate into mature neurons in 2 weeks. HNPs did not express nestin and differ

Plant tissue biosensors for the determin
Plant tissue biosensors for the determination of biogenic diamines and of their amino acid precursors: effect of carbonic anhydrase
✍ Francesco Botrè; Claudio Botrè; Giampiero Lorenti; Franco Mazzei; Fernando Porce 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 507 KB

A new class of plant tissue biosensors has been set up and applied to the determination of biogenic diamines. All the biosensors presented in this work are based on the amperometric determination of H,02 produced by the enzymatic oxidation of diamines (putrescine and cadaverine) by the diamine oxida

Isolation of c-myc genes from goldfish a
Isolation of c-myc genes from goldfish and their tissue specific expression
✍ H. Zhang; K. Futami; N. Okamoto 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 172 KB

One novel cellular __myc__ gene (c‐__myc__), __GM__1, was isolated from a tetraploid cyprinid, the goldfish __Carassius auratus__. __GM__1 consisted of one non‐coding exon and two coding exons, presumably encoding a protein of 390 amino acid (aa) residues. The aa identity between __GM__1 and __GM__2