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Isolation and characterization ofPseudomonas aeruginosamutants deficient in the utilization of the terpenoid citronellic acid

✍ Scribed by M. L. Hector; M. F. Murphy-Waldorf; T. B. Giertych; M. J. Hickey; A. A. Haggard

Publisher
Springer
Year
1993
Tongue
English
Weight
448 KB
Volume
9
Category
Article
ISSN
1573-0972

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✦ Synopsis

Eighteen non-sibling mutants of Pseudomonas aeruginosa PAO were isolated that were deficient in the utilization of the Ξ²-methyl branched acid citronellic acid but not in the utilization of the unbranched n-octanoic acid (Cau mutants). These mutants are also deficient in the utilization of citronellol and citronellal. R68.45 plasmid-mediated transfer of chromosomal material has been used to map one of the mutations at about 52 min on the PAO chromosome and to show linkage of some, but not all, of the other mutations to this region. This system is of interest for bioremediation in oil spill areas since Ξ²-methyl branches block normal Ξ²-oxidation and cause recalcitrance of organic molecules present in petroleum products.

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