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Isolation and Characterization of theKlHEM1 Gene inKluyveromyces lactis

✍ Scribed by González-Domínguez, Mónica; Méndez-Carro, Cristina; Esperanza Cerdán, M.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
197 KB
Volume
13
Category
Article
ISSN
0749-503X

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✦ Synopsis

The KlHEM1 gene from Kluyveromyces lactis encodes a functional 5-aminolevulinate synthase ( ALA synthase), as confirmed by complementation of a hem1 mutant Saccharomyces cerevisiae strain, homology search, and detection of a 2•3 kb transcript. The gene is highly homologous to the ScHEM1 gene, and the sequence of the promoter region contains a complex combination of putative regulatory signals. Some of them are related to phospholipid biosynthesis, glycolytic metabolism, and regulation by carbon source. Transcription of KlHEM1 increased significantly in response to limited oxygen, and only slightly with the change from repressed (glucose) to derepressed conditions (glycerol). The ALA synthase from K. lactis contains, in the amino-terminal region, two hemeresponsive elements that are not present in the protein from Saccharomyces cerevisiae. The complete nucleotide sequence has been entered in the EMBL data library under Accession Number X92944.

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