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Cloning and characterization of theKluyveromyces lactis homocysteine synthase gene

✍ Scribed by Brzywczy, Jerzy; Paszewski, Andrzej

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
372 KB
Volume
15
Category
Article
ISSN
0749-503X

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✦ Synopsis

The Kluyveromyces lactis homocysteine synthase gene was cloned by complementation of the Saccharomyces cerevisiae met25 mutation. The coding sequence of the K. lactis gene shows a high similarity to the S. cerevisiae gene. Very little similarity is found in the 5 and 3 untranslated regions. However, one finds short DNA stretches in the promoter of the K. lactis gene which are identical to the nucletide sequences implicated in the regulation of the S. cerevisiae homologue. This could explain strong transcriptional inhibition of the K. lactis gene by exogenous methionine in the S. cerevisiae host, and indicates a substantial conservation of the sulphur regulatory system between both yeast species.

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