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Isolation and characterization of theCandida albicans SEC4 Gene

✍ Scribed by Clément, Martin; Fournier, Hélène; De Repentigny, Louis; Belhumeur, Pierre

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
190 KB
Volume
14
Category
Article
ISSN
0749-503X

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✦ Synopsis

The SEC4 gene product is a major component of the protein secretion machinery. More specifically, it is believed to play a pivotal role in targeting and fusion of secretory vesicles to the plasma membrane. Its recently described implication with the Saccharomyces cerevisiae Rho3p, which is required for directing growing points during bud formation, has prompted us to investigate the role and function of Sec4p in the morphological changes of the yeast pathogen Candida albicans. We have therefore cloned the C. albicans SEC4 gene. It encodes a 210 amino acids long protein sharing up to 75% homology to the S. cerevisiae homolog, when conserved changes are allowed. Its RNA is constitutively expressed in C. albicans grown under various physiological conditions. We also show that it can functionally complement a S. cerevisiae sec4 thermosensitive mutant.

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