Isolation and characterization of pLS 1 plasmid mutants with increased copy numbers
✍ Scribed by Paloma Acebo; María Teresa Alda; Manuel Espinosa; Gloria del Solar
- Book ID
- 116131939
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 642 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0378-1097
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📜 SIMILAR VOLUMES
Site-specific deletions and insertions in the replication region of plasmid R1 have generated a new class of copy mutants that are present in the cell with 10-15-fold increased copy number. All mutations described inactivate a copy number control gene which is distinct from another cop inc gene that
Copy number (Cop) mutants of miniR6-5 and miniF plasmid derivatives containing a beta-lactamase gene were isolated by selection for increased ampicillin-resistance. Mutants which exhibited an increased copy number and a reduced incompatibility response as compared to the respective parent mini-plasm