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Isolation and characterization of mutants of the RP4 plasmid coding for increased resistance to ampicillin

✍ Scribed by K. Čejka; I. Holubová; J. Hujbáček; O. Navrátil; H. Braná


Book ID
112941713
Publisher
Springer Netherlands
Year
1985
Tongue
English
Weight
372 KB
Volume
30
Category
Article
ISSN
0015-5632

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✍ L. Giudice; D. R. Massardo; F. Manna; K. Wolf 📂 Article 📅 1986 🏛 Springer-Verlag 🌐 English ⚖ 200 KB

Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is res