Isolation and preliminary characterization of Escherichia coli mutants resistant to lethal action of the bacteriophage λ P gene
✍ Scribed by Sarbani Maiti; Biswadip Das; Nitai C. Mandal
- Book ID
- 113209509
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 216 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0042-6822
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The location of 14 independently isolated spontaneous pi A and pi B point mutants in the lambda P gene and their base exchanges were determined. It was found that the pi B mutation is one unique type mapping close to other pi A mutants. The number of possible pi A mutation sites could be estimated.
In order to select mutants that would help to characterize the post-transcriptional regulation of rpsA, we constructed a strain in which the growth rate on lactose minimal medium is determined by the amount of an rpsA-lacZ' alpha-fragment fusion protein produced, even when this is encoded by a high-