Isolation and characterization of a higher-copy-number mutant of plasmid R6K
✍ Scribed by J. Nešvera; J. Hochmannová
- Book ID
- 112924792
- Publisher
- Springer Netherlands
- Year
- 1983
- Tongue
- English
- Weight
- 771 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0015-5632
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Copy number (Cop) mutants of miniR6-5 and miniF plasmid derivatives containing a beta-lactamase gene were isolated by selection for increased ampicillin-resistance. Mutants which exhibited an increased copy number and a reduced incompatibility response as compared to the respective parent mini-plasm
Site-specific deletions and insertions in the replication region of plasmid R1 have generated a new class of copy mutants that are present in the cell with 10-15-fold increased copy number. All mutations described inactivate a copy number control gene which is distinct from another cop inc gene that