Variants resistant to 2-deoxy-D-glucose have been isolated from a clonal line of pig kidney cells by serial cultivation in the presence of inhibitor. Hexokinase activity may be affected directly i n this system, since the oxidation of glucose to 6-phosphogluconate by extracts from sensitive and resi
Isolation and characterization of mutants of Trichosporon adeninovorans resistant to 2-deoxy-D-glucose
✍ Scribed by Dr. R. Büttner; A. Scheit; R. Bode; D. Birnbaum
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 346 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
We isolated stable mutants from Trichosporon adeninovorans resistant to 2-deoxy-~-glucose. All mutants were altered in the C-catabolite repression and produced different amounts of glucoamylase and 8-glucosidase when grown on several carbon sources. In addition to the C-catabolite derepression of these extracellular enzymes, a repression defect .of isocitrate lyase was found.
Several yeast species have been investigated as potentially valuable candidates for the production of single cell protein or ethanol from starch. Therefore, in various laboratories extracellular amylolytic enzymes of yeasts have become subject of study (CLEMENTI et al.
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