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Isolation and characterization of mitochondrial DNA of the oomycetous fungusPhytophthora infestans

✍ Scribed by L. J. Klimczak; H. H. Prell

Publisher
Springer-Verlag
Year
1984
Tongue
English
Weight
336 KB
Volume
8
Category
Article
ISSN
0172-8083

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✦ Synopsis

Mitochondrial DNA (mtDNA) of Phytophthora infestans has been isolated and preliminarily characterized. It has a low GC content of about 22.4% which is distinctly different from that of nuclear DNA (51%). This property has been used to separate both DNA species in the presence of 4',6-diamidine-2-phenylindole (DAPI) in CsC1 density gradients. The use of cetyltriammonium bromide (CTAB) for extraction of DNA significantly reduced its degradation. The base distribution of the mtDNA shows a limited intramolecular heterogeneity. The molecule contains 36.2 -+ 0.3 kb as revealed by endonuclease digestion and seems to be circular as shown by restriction mapping. No differences were found in restriction patterns between mtDNAs from various pathotypes.

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