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Isolation and characterization of mitochondrial DNA from the alkane yeastSaccharomycopsis lipolytica

✍ Scribed by U. Kück; U. Stahl; A. Lhermitte; K. Esser

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
630 KB
Volume
2
Category
Article
ISSN
0172-8083

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✦ Synopsis

Mitochondrial (mt) DNA of the alkane yeast, Saecharomyeopsis lipolytiea, was isolated. Its buoyant density in CsC1 was found to be of 1.687 g/cm 3 , indicating a GC content of 27.5% and its melting point T m = 79.5 °C, indicating a GC content of 24.9%. The corresponding values for nuclear (n) DNA, are 1.709 g/cm 3 (GC: 49.5%) and T m = 90.5 (GC:51.7%) respectively. Electron microscopy revealed that mtDNA has a circular structure with a contour length of about 14.5 /lm corresponding to 45.5 kb per molecule. The size estimated from restriction analyses performed with 7 endonucleases was 48.35'kb/ molecule. A restriction map was constructed, using the cleavage data of 4 endonucleases.

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