Cloning and characterization of the peroxisomal acyl CoA oxidaseACO3 gene from the alkane-utilizing yeastYarrowia lipolytica

✍ Scribed by Wang, Huijie; Le Clainche, Annick; Le Dall, Marie-Thérèse; Wache, Yves; Pagot, Yves; Belin, Jean-Marc; Gaillardin, Claudie; Nicaud, Jean-Marc

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 421 KB
• Volume: 14
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(199811)14:15<1373::aid-yea332>3.0.co;2-1

✦ Synopsis

The ACO3 gene, which encodes one of the acyl-CoA oxidase isoenzymes, was isolated from the alkane-utilizing yeast Yarrowia lipolytica as a 10 kb genomic fragment. It was sequenced and found to encode a 701-amino acid protein very similar to other ACOs, 67.5% identical to Y. lipolytica Aco1p and about 40% identical to S. cerevisiae Pox1p. Haploid strains with a disrupted allele were able to grow on fatty acids. The levels of acyl-CoA oxidase activity in the ACO3 deleted strain, in an ACO1 deleted strain and in the wild-type strain, suggested that ACO3 encodes a short chain acyl-CoA oxidase isoenzyme. This narrow substrate spectrum was confirmed by expression of Aco3p in E. coli.