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Molecular characterization and cloning of sheep mitochondrial DNA

✍ Scribed by Michel Villalta; Patricio Fernández-Silva; Belén Beltrán; Luisa Enguita; Manuel J. López-Pérez; Julio Montoya

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
835 KB
Volume
21
Category
Article
ISSN
0172-8083

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✦ Synopsis

Mitochondrial DNA from the liver of a single Rasa Aragonesa sheep has been isolated and characterized. The size of the genome, determined by restriction enzyme analysis, was found to be 16.58 kbp. The cleavage sites for the restriction endonucleases BamHI, HindIII, EcoRI, BglII, PvuII, BstEII and PstI were mapped, and the gene organization deduced through heterologous hybridization using different cloned fragments of the rat mitochondrial genome. Fragments representative of the entire sheep genome were cloned in plasmid vectors pGEM3Z and pUN121.

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