Isolation and characterization of lipophorin from Drosophila melanogaster larvae

✍ Scribed by Germain J. P. Fernando-Warnakulasuriya; Dr. Michael A. Wells

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 369 KB
• Volume: 8
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/arch.940080405

✦ Synopsis

The hemolymph lipoprotein lipophorin has been isolated from third-instar Drosophila melanogaster larvae by a technique that involves homogenization of whole larvae in a medium containing protease inhibitors and purification of the lipoprotein by density gradient centrifugation. Drosophila lipophorin has a density of 1.16 g/ml and is composed of 62.5% protein, 23.1% phospholipid, 7.4% diacylglycerol, 5.4% triacylglycerol, 0.9% hydrocarbon, and 0.7% sterol. As is the case with other insect lipophorins, Drosophila lipophorin contains two apolipoproteins, apolipophorin-l (M, = 275,000) and apolipophorin-l I (M, = 76,000). Drosophila apolipophorin-l does not crossreact with antibodies prepared against apolipophorin-l from Manduca sexta.