Characterization of lipophorin from Antheraea mylitta drury (Lepidoptera: Saturniidae)
✍ Scribed by P.P. Srivastava; K. Thangavelu
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 417 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0739-4462
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✦ Synopsis
The high molecular weight (680 KDa) glycolipoprotein from the haemolymph of male larvae of Antheraea mylitta Drury (Lepidoptera: Saturniidae) was identified as lipophorin by gradient KBr ultracentrifugation and SDS-PAGE. This lipophorin is composed of two subunits: apolipoprotein I (234 KDa) and apoprotein II (80 KDa). The density of the native molecule is 1 .I941 g/ml. By weight, it contains 53.7% protein, 3.7% carbohydrate, and 42.6% lipid. Neutral lipids and phospholipids are 66.2 and 33.8% of the total lipids, respectively. Mannose and N-acetylglucosamine are the only sugars detected by gas liquid chromatography. The amino acid composition of both the native molecule and its two subunits was determined, and yielded similar amino acid COmpOSitiOns.