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Isolation and characterization of high-mobility-group proteins from maize

✍ Scribed by Klaus D. Grasser; Andreas Wurz; Günter Feix

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
1012 KB
Volume
185
Category
Article
ISSN
0032-0935

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✦ Synopsis

Chromosomal nonhistone high-mobility-group (HMG) proteins were purified from nuclei of maize (Zea mays L. cv. A619) endosperm and leaf tissue. Tissuespecific differences were observed in their polypeptide patterns, in in-vitro phosphorylation experiments with a casein-kinase type II, and by Western blot analysis with antisera against different HMG proteins. Gelfiltration chromatography demonstrated that maize HMG proteins occur as monomers. By measuring the capacity of the HMG proteins to bind to the 5' flanking region of a zein gene, the sensitivity of the proteins to different temperatures, salt concentrations and pH values was determined.

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