Isolation and partial characterization of feruloylated oligosaccharides from maize bran
✍ Scribed by Luc Saulnier; Jacqueline Vigouroux; Jean-François Thibault
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 608 KB
- Volume
- 272
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Maize bran contains phenolic acids [ ~ 4% dry matter; mainly ferulic acid (Fe) and also diferulic acid], heteroxylans (~ 50%), and cellulose (~ 20%), but is devoid of lignin. Treatment of maize pericarp with 0.05 M trifluoroacetic acid at 100°C for 2 h released ~ 90% of the heteroxylans and ~ 90% of the ferulic acid and its esters. After fractionation of the products with Amberlite XAD-2 and Sephadex LH-20 three main feruloylated oligosaccharides (F3-F 7) were isolated. They represented ~ 30% of the ferulic acid, and ~ 2% of the neutral sugars contained in the hydrolysis supernatant.
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## Abstract A method for the isolation of diferulic and triferulic acids in quantities and purity that comply with the requirements for their use as standard substances was developed. The procedure includes alkaline hydrolysis of destarched maize bran and ether extraction of liberated phenolic comp