Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines expre
β¦ LIBER β¦
Isolation and characterization of chemically transformed pancreatic acinar cell lines from young and old mice
β Scribed by Karen Zelinsky-Papez; Timothy H. Carter; Jay A. Zimmerman
- Book ID
- 112799784
- Publisher
- Society for In Vitro Biology
- Year
- 1987
- Tongue
- English
- Weight
- 466 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1475-2689
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## Abstract 3T3 mouse fibroblasts in 10% calf serum cease to increase in number at confluence. SV40βtransformed 3T3 cells lose their sensitivity to this growth control and so are able to reach multilayered cell densities. We previously described a polyploid revertant line F1SV101, isolated from a S