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Isolation and characterization of alkaline phosphatase-constitutive variants from chinese hamster ovary cells (CHO-K1)

✍ Scribed by Kiyoshi Nose; Hajim Katsuta

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
489 KB
Volume
86
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Pure clones with high alkaline phosphatase (ALP) activity were isolated from Chinese hamster ovary (CHO‐K1) cells which showed no detectable ALP activity. ALP‐positive cells appeared at the frequency of 10^−4^ in the N‐methyl‐N'‐nitro‐N‐nitrosoguanidine‐treated cell population.

With respect for cellular morphology, plating efficiency and several other enzymatic activities, no distinct difference was found between the original CHO‐K1 cells and its ALP‐positive variants, although some alterations in karyotype were found.

Levels of ALP activity in these clones was stably maintained during serial cultivation. Some of the enzymological properties of ALP in the isolated clones were similar to those in bone or kidney.

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