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Isolation and characterization of a recA mutant of Erwinia chrysanthemi B374

✍ Scribed by Faelen, Michel ;Schoonejans, Eric ;Toussaint, Ariane

Publisher
Springer
Year
1985
Tongue
English
Weight
323 KB
Volume
201
Category
Article
ISSN
0026-8925

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✦ Synopsis

Cosmids carrying a rec gene of the Erwinia chrysanthemi strain B 374 were identified in a genomic library constructed by Reverchon et al. (1985). One of them was mutagenized with transposon Tnl and the rec: : T n l mutation was introduced into the B 374 chromosome, providing the first rec-less E. chrysanthemi strain. The pectinolytic and cellulolytic activities were unaffected by the rec mutation.

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