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The cya gene region of Erwinia chrysanthemi B374: organisation and gene products

✍ Scribed by Hedegaard, Lisbeth ;Danchin, Antoine

Publisher: Springer
Year: 1985
Tongue: English
Weight: 560 KB
Volume: 201
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00397984

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✦ Synopsis

A DNA fragment containing the cya gene region ofErwinia chrysanthemi, B374 was cloned in vivo and transferred into cells of E. coli using a plasmid pULB113 derived from RP4 followed by subcloning in vitro into the vector pBR322. The cya gene encodes a 95 kDal protein that complemented E. coli cya mutants. Apparently, cya genes truncated at the Yend could still produce proteins complementing cya-defective strains, thus showing that adenylate cyclase truncated at its carboxy-terminal end could synthesise cAMP. A protein of unknown function (40 kDal) is encoded by a gene that is transcribed divergently from the control region of the adenylate cyclase gene.

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