We have developed a simple and rapid method for isolation of purified nuclear lamina from Ehrlich ascites tumor cells. The procedure employs chromatin structures prepared from whole cells at low ionic strength and is carried out under conditions that minimize the formation of artifactual protein-DNA
โฆ LIBER โฆ
Isolation and characterization of a family of .alpha.-D-galactosyl-containing glycopeptides from Ehrlich ascites tumor cells
โ Scribed by Eckhardt, Allen E.; Goldstein, Irwin J.
- Book ID
- 125934920
- Publisher
- American Chemical Society
- Year
- 1983
- Tongue
- English
- Weight
- 955 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-2960
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Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and aff