Isolation and partial characterization of the ADP-ribosylated nuclear proteins from Ehrlich ascites tumor cells
โ Scribed by Peter Adamietz; Karin Klapproth; Helmuth Hilz
- Book ID
- 118313564
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 1007 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
We have developed a simple and rapid method for isolation of purified nuclear lamina from Ehrlich ascites tumor cells. The procedure employs chromatin structures prepared from whole cells at low ionic strength and is carried out under conditions that minimize the formation of artifactual protein-DNA
Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and aff