Isoelectric focusing in soil science
β Scribed by Francesco Alianiello
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 318 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1615-9306
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β¦ Synopsis
Abstract
Applications of isoelectric focusing (IEF) in soil science are illustrated. The materials studied, i.Β e. humic substances and organic fertilisers, and their behaviour when subjected to IEF are briefly described. Preliminary studies carried out on soil indicate a relation between the isoelectric point and molecular weight, and a connection with humification has been hypothesised; therefore, assessment of soil conditions could be an application of IEF. Another application is evaluation of the stabilisation of organic fertilisers. Finally, IEF can be used to distinguish different organic matrices, as such and in mixtures with mineral fertilisers.
π SIMILAR VOLUMES
An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approx
Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane