Isoelectric focusing of brain adenylate cyclase

✍ Scribed by Douglas J. Franks; Daniel Malamud

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 392 KB
• Volume: 73
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90198-6

✦ Synopsis

Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. The enzyme activity has been measured with a sensitive assay isolating cyclic AMP from Dowex and alumina columns. The technique allows a one-step analysis of this membrane enzyme from a heterogeneous sample within 6 hr.