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Isoelectric focusing in a Sephadex column

✍ Scribed by T.J. O'Brien; H.H. Liebke; H.S. Cheung; L.K. Johnson

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
393 KB
Volume
72
Category
Article
ISSN
0003-2697

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✦ Synopsis

An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approximately 5 hr and thereby reduces the exposure of more labile proteins to long focusing times.

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## Abstract Applications of isoelectric focusing (IEF) in soil science are illustrated. The materials studied, i.Β e. humic substances and organic fertilisers, and their behaviour when subjected to IEF are briefly described. Preliminary studies carried out on soil indicate a relation between the iso