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Inﬂuence of nonionic surfactants on the chromatographic behaviour of proteins in hydrophobic interaction chromatography

✍ Scribed by Marina D. Rukhadze; Maya V. Sebiskveradze; Teona G. Makharadze; Nino Sh. Sidamonidze

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 118 KB
Volume: 17
Category: Article
ISSN: 0269-3879
DOI: 10.1002/bmc.282

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✦ Synopsis

Abstract

The chromatographic characteristics of proteins in the presence of additives of nonionic surfactants Brij‐35 and Tween‐80 in the conditions of descending gradient of ammonium sulfate and phenyl‐coated polymeric stationary phase were investigated. It was revealed that retention factors of proteins may be regulated by use of mentioned additives. The improvement of resolution is achieved for some hardly separated pairs of proteins, viz. albumin egg/albumin bovine, aldolase/tripsin. A reversion of the elution order is observed for tripsin/chymotrypsinogen A. Copyright © 2003 John Wiley & Sons, Ltd.

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