The Na / pump (Na / , K / -ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na / pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monit
Involvement of Na+, K+-ATPase inhibition in K562 cell differentiation induced by bufalin
β Scribed by Satoshi Numazawa; Masa-Aki Shinoki; Hisatomi Ito; Takemi Yoshida; Yukio Kuroiwa
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 847 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0021-9541
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β¦ Synopsis
Human leukemia K562 cell differentiation induction by naturally occurring bufadienolides purified from the Chinese drug Senso and synthetic bufalin derivatives was examined by a nitro blue tetrazolium reduction assay. Bufalin showed
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MDCK kidney epithelial cell cultures exposed to the differentiation inducer hexamethylene bisacetamide (HMBA) for 24 hours exhibited a 50% decrease in transport activity per (Na+, K+)-ATPase molecule (turnover number) but an unchanged number of pump sites (Kennedy and Lever, 1984). Inhibition of pro
Treatment of Friend erythroleukemia cells with several different chemical agents causes an early decrease in the 86 Rb+ influx mediated by Na+/K+ adenosine triphosphatase (ATPase). These agents, which induce Friend cells t o differentiate, include dimethylsulfoxide (DMSO), ouabain, hypoxanthine, and
The ATPase activity in human red blood cell membranes was investigated in vitro as a function of temperature and exposure to 2,450-MHz continuous wave microwave radiation to confirm and extend a report of Na+ transport inhibition under certain conditions of temperature and exposure. Assays were cond
To gain insight into the mechanisms responsible for differentiation of hippocampal neurons growing in vitro, the effects of estrogen on neuritic development and on activity and distribution of isoforms of the Na,K-ATPase, were evaluated. Dissociated cells from hippocampi of 19-day-old rat fetuses we
## Abstract Na^+^,K^+^βATPase, a basolateral transporter responsible for tubular reabsorption of Na^+^ and for providing the driving force for vectorial transport of various solutes and ions, can also act as a signal transducer in response to the interaction with steroid hormones. At nanomolar conc