Temperature-specific inhibition of human red cell Na+/K+ ATPase by 2,450-MHz microwave radiation
✍ Scribed by John W. Allis; Barbara L. Sinha-Robinson
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 693 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0197-8462
No coin nor oath required. For personal study only.
✦ Synopsis
The ATPase activity in human red blood cell membranes was investigated in vitro as a function of temperature and exposure to 2,450-MHz continuous wave microwave radiation to confirm and extend a report of Na+ transport inhibition under certain conditions of temperature and exposure. Assays were conducted spectrophotometrically during microwave exposure with a custom-made spectrophotometer-waveguide apparatus. Temperature profiles of total ATPase and Ca" ATPase (ouabain-inhibited) activity between 17 and 31 "C were graphed as an Arrhenius plot. Each data set was fitted to two straight lines which intersect between 23 and 24 "C. The difference between the total and C a f Z ATPase activities, which represented the Na+/K+ ATPase activity, was also plotted and treated similarly to yield an intersection near 25 "C. Exposure of membrane suspensions to electromagnetic radiation, at a dose rate of 6 W/kg and at five temperatures between 23 and 27 "C, resulted in an activity change only for the Na+/K' ATPase at 25 "C. The activity decreased by approximately 35 % compared to sham-irradiated samples. A possible explanation for the unusual ternperature/microwave interaction is proposed.