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The program of friend cell erythroid differentiation: Early changes in Na+/K+ ATPase function

✍ Scribed by Mager, Dixie ;Bernstein, Alan

Publisher: Wiley (John Wiley & Sons)
Year: 1978
Tongue: English
Weight: 522 KB
Volume: 8
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400080405

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✦ Synopsis

Treatment of Friend erythroleukemia cells with several different chemical agents causes an early decrease in the 86 Rb+ influx mediated by Na+/K+ adenosine triphosphatase (ATPase). These agents, which induce Friend cells t o differentiate, include dimethylsulfoxide (DMSO), ouabain, hypoxanthine, and actinomycin D. The magnitude of the early decrease in 86 Rb' influx correlates with the proportion of cells in cultures of inducible Friend cell clones which later go o n to synthesize hemoglobin. Compounds which do not incude differentiation in these cells, such as xanthine, exogenous hematin, and erythropoietin, do not cause a change in 86 Rb+ influx. A change in the intracellular K+ ion concentration does not occur during induction by DMSO because, although there is a decrease in K+ content per cell soon after induction, there is a parallel decrease in cell volume. These results and previous observations from this laboratory are discussed in terms of the posible involvement of the Na+/K+ ATPase in Friend cell differentiation.

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