1,25-dihydroxyvitamin D3 selectively induces increased expression of the Na,K-ATPase β1 subunit in avian myelomonocytic cells without a concomitant change in Na,K-ATPase activity
✍ Scribed by Agnes Billecocq; William C. Horne; Munmun Chakraborty; Kunio Takeyasu; Robert Levenson; Roland Baron
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 219 KB
- Volume
- 172
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Treatment of avian myelomonocytic cells with 1,25-dihydroxyvitamin D 3 (1,25(OH)2D3) results in an approximately two fold increase in levels of Na,K-ATPase b1 subunit mRNA and protein (both total and plasma membrane-associated). The changes in b1 subunit expression occur in the absence of a detectable increase in expression of any of the three a subunit isoforms or in Na,K-ATPase activity. The selective induction of the expression of the b subunit in avian myelomonocytic cells by 1,25(OH)2D3 reveals a previously unobserved feature of the regulation of Na,K-ATPase expression, while the targeting of b subunit polypeptides to the plasma membrane in the absence of a corresponding increase in active Na,K-ATPase suggests that, in these cells, transport of the b subunit to the plasma membrane may be independent of its binding to the a subunit.