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Investigation of the Ligand-binding Mechanism of Methionine Sulfoxide Reductase A of E.coli

✍ Scribed by Smith, Virginia F.; Kesireddy, Nikhil; So, Joanne D.

Book ID
122624272
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
41 KB
Volume
96
Category
Article
ISSN
0006-3495

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A simple and rapid assay has been developed to measure the enzymatic activity of peptide methionine sulfoxide reductase. The assay is based on the reduction of labeled N-acetylmethionine sulfoxide to N-acetvlmethionine. The N-acetylmethionine can be separated from the substrate by extraction into et