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Diastereoselective reduction of protein-bound methionine sulfoxide by methionine sulfoxide reductase

✍ Scribed by Victor S. Sharov; Deborah A. Ferrington; Thomas C. Squier; Christian Schöneich


Book ID
117111160
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
126 KB
Volume
455
Category
Article
ISSN
0014-5793

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A simple and rapid assay has been developed to measure the enzymatic activity of peptide methionine sulfoxide reductase. The assay is based on the reduction of labeled N-acetylmethionine sulfoxide to N-acetvlmethionine. The N-acetylmethionine can be separated from the substrate by extraction into et