Investigation of methods for measurement of radioactivity in tritiated DNA and applications to assays for DNA polymerase activity

✍ Scribed by Bruce K. Schrier; Samuel H. Wilson

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 716 KB
• Volume: 56
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(73)90184-x

✦ Synopsis

Methods for collection and counting of 'H-labeled DNA on nitrocellulose and glass fiber filters have been investigated. The findings are of potential importance in determining the amount of radioactivity in DNA as well as other macromolecules.

The highest counting efficiencies were observed using glass fiber filters, NCS for dissolving DNA, and a toluene scintillation mixture for counting. However, glass fiber filters, even with large amounts of co-precipitant albumin, failed to collect all of low molecular weight (appro'x 185,000 daltons) DNA. Thus, in many applications nitrocellulose filters proved to be more advantageous.