Automated fluorometric analysis of DNA, protein, and enzyme activities: Application to methods in cell culture

✍ Scribed by James W. Kurtz; William W. Wells

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 744 KB
• Volume: 94
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90806-6

✦ Synopsis

Automated fluorometric methods for the analysis of DNA, protein, and selected enzyme activities for N-acetyt-P-D-glucosaminidase, glucose 6-phosphate dehydrogenase, and 6phosphogluconate dehydrogenase are described. Instrumentation for these assays includes a Gilford 3500 computer-directed analyzer in conjunction with a Farrand Ratio Fluorometer-2 modified for flowthrough sampling. Comparisons were made between the automated fluorometric methods described and manual spectrophotometric or fluorometric methods for reproducibility, speed of analysis, and quantitative correlation. Typical values of Nacetyl-/3-D-ghrcosaminidase, glucose 6-phosphate dehydrogenase, and 6phosphogluconate dehydrogenase activities obtained by these methods in isolated rat hepatocytes and Reuber H-35 hepatoma cells are reported.