Interspecific protoplast fusion of the Baker's yeastSaccharomyces cerevisiaeandSaccharomyces diastaticus

✍ Scribed by L. I. Figueroa; M. F. Richard; M. R. Broock

• Publisher: Springer Netherlands
• Year: 1984
• Tongue: English
• Weight: 286 KB
• Volume: 6
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00140049

✦ Synopsis

The protoplast fusion technique provides a useful method For improving industrial yeasts and a~olutinant anents like polyethylene qlycol (PEP) MY! 4000 and Ca ++ ions are widely used to stimulate the fusion process. Commercial Baker's yeast Soc~omwce3 c~euX3iae and Sacchc~omycEs dx~a6Y~uticcu~ were selected as parental strains for somatic fusion. The S~cch~ytom#c~ ~oL6ta~c{~ carried a spontaneous petite mutation and could not metabolize starch unlike respiratory comDetent Sacchoptomuc~ cL~eu3/Ccci~[cuy3 From which it was derived, that readily could A medium containing soluble starch as a carbon source and 3% afar was used as fusion products selection medium. Respiratory competent fusion products were capable of usin~ dextrins and starch as carbon source~.