Interspecific protoplast Fusion of Schwanniomyces yeasts
β Scribed by Motiram R. Dhawale; W. Michael Ingledew
- Publisher
- Springer Netherlands
- Year
- 1983
- Tongue
- English
- Weight
- 337 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0141-5492
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Hypocotyl derived protoplasts of B. juncea cv. RLM-198 were fused with mesophyll protoplasts of B. spinescens using polyethylene glycol to produce interspecific hybrids. Fusion products could be microscopically identified by characteristics of the protoplasts of both parents in the hybrid cells; the
Saccharomyces cerevisiae hisl trp2 resistant to acriflavine and able to ferment galactose and of Saccharomyces fermentati arg resistant to DL-pfluorophenylalanine and able to ferment lactose were fused. As a result of fusion two types of prototrophic hybrids were obtained. Type 1 hybrids were able t
The protoplast fusion technique provides a useful method For improving industrial yeasts and a~olutinant anents like polyethylene qlycol (PEP) MY! 4000 and Ca ++ ions are widely used to stimulate the fusion process. Commercial Baker's yeast Soc~omwce3 c~euX3iae and Sacchc~omycEs dx~a6Y~uticcu~ were