Intersectin1-s is involved in migration and invasion of human glioma cells

✍ Scribed by Yongjie Ma; Bingbing Wang; Wenliang Li; Xiaoli Liu; Jing Wang; Ting Ding; Jiao Zhang; Guoguang Ying; Li Fu; Feng Gu

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 709 KB
• Volume: 89
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.22616

✦ Synopsis

Abstract

Malignant gliomas have a tendency to invade diffusely into surrounding healthy brain tissues, thereby precluding their successful surgical removal. Intersectin1 (ITSN1) as a molecular linker in the central nervous system is well known as an important regulator of endocytosis and exocytosis. ITSN1 has two isoforms: ITSN1‐l and ITSN1‐s. In this study, we show that siRNA‐mediated down regulation of ITSN1‐s inhibited migration and invasion of glioma cells. In addition, we demonstrate the possible mechanisms by which ITSN1‐s functions in migration and invasion. Several key proteins, including cofilin, LIMK, PAK, FAK, integrin β1, and MMP‐9, which are critical for cells migration and invasion, were probably involved in ITSN1‐s signaling pathways. These results suggest that ITSN1‐s contributes to glioma cells migration and invasion by regulating the formation of cytoskeleton, influencing adhesion and increasing expression of MMP‐9. Our results indicate that ITSN1‐s is a critical factor in glioms invasion and identify that ITSN1‐s is a new potentially antiinvasion target for therapeutic intervention in gliomas. © 2011 Wiley‐Liss, Inc.